We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Imipenem resistance in clinical <italic>Escherichia coli</italic> from Qom, Iran.
- Authors
Shams, Saeed; Hashemi, Ali; Esmkhani, Mohammad; Kermani, Somaye; Shams, Elham; Piccirillo, Alessandra
- Abstract
Objective: The emergence of metallo-β-lactamase-producing <italic>Enterobacteriaceae</italic> is a worldwide health concern. In this study, the first evaluation of MBL genes, <italic>bla</italic><italic>IMP</italic> and <italic>bla</italic><italic>VIM</italic>, in <italic>Escherichia coli</italic> resistant to imipenem isolated from urine and blood specimens in Qom, Iran is described. Three hundred urine and blood specimens were analysed to detect the presence of <italic>E. coli</italic>. Resistance to imipenem and other antimicrobials was determined by disk diffusion and MIC. MBL production was screened using CDDT. PCR was also carried out to determine the presence of <italic>bla</italic><italic>IMP</italic> and <italic>bla</italic><italic>VIM</italic> genes in imipenem-resistant isolates. Results: In total, 160 <italic>E. coli</italic> isolates were collected from March to May 2016. According to disk diffusion, high-level of resistance (20%) to cefotaxime was observed, whereas the lowest (1%) was detected for tetracycline. In addition, five isolates showed resistance to imipenem with a MIC ≥ 4 µg/mL. CDDT test confirmed that five isolates were MBL-producing strains, but no <italic>bla</italic><italic>IMP</italic> and <italic>bla</italic><italic>VIM</italic> genes were detected. Results of this study show a very low level of resistance to imipenem in our geographical area.
- Subjects
BETA lactamases; IMIPENEM; ENTEROBACTERIACEAE; ESCHERICHIA coli; GENES
- Publication
BMC Research Notes, 2018, Vol 11, Issue 1, pN.PAG
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/s13104-018-3406-6