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- Title
Sperm chromatin protamination influences embryo development in unsexed and sexed bull semen.
- Authors
Silva, Thiago Velasco Guimarães; Santana, Priscila Di Paula Bessa; Souza, Eduardo Baia de; Lima, Ana Júlia Mota de; Santos, Caroline de Araújo; Almeida, Nathália Nogueira da Costa; Brito, Vanessa Cunha de; Gonçalves, Arnaldo Algaranhar; Rolim Filho, Sebastião Tavares; Cordeiro, Marcela da Silva; Santos, Simone do Socorro Damasceno; Miranda, Moysés dos Santos; Ohashi, Otávio Mitio
- Abstract
Summary: Sex selection through sperm sorting offers advantages in regards selection pressure in high-producing livestock. However, the sex-sorting process results in sperm membrane and DNA damage that ultimately decrease fertility. We hypothesized that given the role of protamines in DNA packaging, protamine deficiency could account, at least partially, for the DNA damage observed following sperm sex sorting. To test this, we compared protamine status between unsexed and sexed spermatozoa from two bulls using the fluorochrome chromomycin A3 (CMA3) and flow cytometry. Then, we assessed embryo development following in vitro fertilization (IVF) using the same sperm treatments. Overall, sperm protamination was not different between sexed and unsexed semen. However, one of the two bulls displayed higher rates of protamine deficiency for both unsexed and sexed semen (P < 0.05). Moreover, unsexed semen from this bull yielded lower blastocyst (P < 0.05) and blastocyst hatching rates than unsexed sperm from the other bull. CMA3-positive staining was negatively correlated with cleavage (R2 85.1, P = 0.003) and blastocyst hatching (R2 87.6, P = 0.006) rates in unsexed semen. In conclusion, while the sex-sorting process had no effect on sperm protamine content, we observed a bull effect for sperm protamination, which correlated to embryo development rates following IVF.
- Subjects
SEMEN; CHROMATIN; SPERMATOZOA; EMBRYOS; FERTILIZATION in vitro
- Publication
Zygote, 2021, Vol 29, Issue 4, p264
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199420000775