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- Title
R-Loop Formation In Trans at an AGGAG Repeat.
- Authors
Kazuya Toriumi; Takuma Tsukahara; Ryo Hanai
- Abstract
Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease Tl was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.
- Subjects
RNA polymerases; RIBONUCLEASE T1; GENETIC transcription; NUCLEOTIDE sequence; REPEATED sequence (Genetics)
- Publication
Journal of Nucleic Acids, 2013, p1
- ISSN
2090-0201
- Publication type
Article
- DOI
10.1155/2013/629218