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- Title
Two-photon uncaging of γ-aminobutyric acid in intact brain tissue.
- Authors
Matsuzaki, Masanori; Hayama, Tatsuya; Kasai, Haruo; Ellis-Davies, Graham C. R.
- Abstract
We have synthesized a photosensitive (or caged) 4-carboxymethoxy-5,7-dinitroindolinyl (CDNI) derivative of γ-aminobutyric acid (GABA). Two-photon excitation of CDNI-GABA produced rapid activation of GABAergic currents in neurons in brain slices with an axial resolution of approximately 2 μm and enabled high-resolution functional mapping of GABA-A receptors. Two-photon uncaging of GABA, the main inhibitory neurotransmitter, should allow detailed studies of receptor function and synaptic integration with subcellular precision.
- Subjects
GABA; BIOSYNTHESIS; PHOTONS; NEUROTRANSMITTERS; SYNAPSES; NEURONS
- Publication
Nature Chemical Biology, 2010, Vol 6, Issue 4, p255
- ISSN
1552-4450
- Publication type
Article
- DOI
10.1038/nchembio.321