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- Title
Effect of antioxidants on cisplatin-induced cytotoxicity and oxidative stress in colon cancer cells.
- Authors
AlSaady, Teeb Ali; Madlum, Kaiser N.; Obied, Hamid Naji
- Abstract
Many anticancer drugs rely on the generation of intracellular ROS as the main mechanism of action for their cytotoxicity. On the other hand, antioxidants are advised for cancer patients to enhance their immunity. Thus, there must be some sort of regulation to solve this conundrum. This study aimed to investigate the antagonistic interaction between the anticancer drug and the antioxidant agents. Cisplatin was used in this as a ROS generator anticancer agent, while Vitamin C and Glutathione, the most common antioxidants were used as ROS scavengers. Results showed that antioxidants have extremely low cytotoxic effects on cancer cells even at relatively high concentrations. The presence of antioxidants in the medium significantly quenches both native and Cisplatin-induced ROS. The treatments of cancer cells with the (Cisplatin-Antioxidant) combination significantly decrease anticancer activity at higher antioxidants doses. Pretreatment of cancer cells with vitamin C followed by treatment with Cisplatin significantly elevate ROS levels, while combined treatment of both agents at the same time reduce ROS levels near normal values. As a conclusion, antioxidants may interfere with anticancer agents that work via ROS-dependent cytotoxicity when administrated simultaneously.
- Subjects
ANTIOXIDANTS; CISPLATIN; ANTINEOPLASTIC agents; IMMUNITY; COLON cancer; OXIDATIVE stress
- Publication
EurAsian Journal of Biosciences, 2020, Vol 14, Issue 2, p5375
- ISSN
1307-9867
- Publication type
Article