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- Title
Effects of Different Equilibration Times on Post-Thaw Cryopreserved Semen Quality of Cattle and Buffalo Bulls.
- Authors
Khan, Adnan; Khan, Rifat Ullah; Qureshi, Muhammad Subhan; Mobashar, Muhammad; Gohar, Ali; Ahmad, Shakoor; Khan, Kamran; Khan, Hamayun; Naz, Shabana
- Abstract
Nine mature bulls were selected in three (n=9) different groups (Holstein Fresian, Jersey and Nili Ravi) to find out equilibration time (T1, 2 h; T2, 6 h and T3, 6 h) effects on different semen quality parameters. Highest sperm progressive motility, sperm plasma membrane integrity were observed in T2. It reflects high damages at T3 and T1 due to cryo shock and oxidative stress respectively. Sperm DNA integrity and pH were increased linearly from T1 to T3. The dead sperm percentage was significantly (P<0.01) low in groups with semen sample treated with T2 compared to other groups. Significantly (P<0.01) high sperm individual motility percentage was observed for T2 than T3 and T1. Sperm acrosomal integrity percentage showed significantly ((P<0.01) high value in T2. It is concluded that 4 h equilibration time improved semen qualitative characteristics. Among the three breeds, Jersey bull semen samples showed the best quality. Equilibration time of 2 and 6 hours showed detrimental effect on the semen quality.
- Subjects
JERSEY (Channel Islands); SEMEN analysis; BULLS; FROZEN semen; SPERM motility; CATTLE; SEMEN; SPERMATOZOA
- Publication
Pakistan Journal of Zoology, 2021, Vol 53, Issue 2, p613
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/20190824040816