We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Human myoblast transplantation: Preliminary results of 4 cases.
- Authors
Huard, J.; Bouchard, J. P.; Roy, R.; Malouin, F.; Dansereau, G.; Labrecque, C.; Albert, N.; Richards, C. L.; Lemieux, B.; Tremblay, J. P.
- Abstract
Myoblasts from immunocompatible donors have been transplanted into the muscles (tibialis anterior, biceps brachii, and/or extensor carpi radialis longus) of 4 Duchenne patients in the advanced stages of the disease. Although no immunosuppressive treatment was used, none of the patients showed any clinical signs of rejection such as fever, redness, and inflammation. One patient transiently produced antibodies against the donor myoblasts as determined by cytofluorometric analysis. This patient and 2 others were shown to form antibodies against their donor's myotubes. Muscle biopsies of the injected tibialis anterior of 4 patients revealed that 80%, 75%, 25% and 0% of the muscle fibers, respectively, showed some degree of dystrophin immunostaining, The contralateral noninjectd muscles of the latter 3 patients did not contain any dystrophin positive fibers, while that of the first patient showed dystrophin expression in 16% of the fibers examined. Myoblasts were also injected into the extensor carpi radialis longus or the biceps brachii of these patients. A few months subsequent to injection, one patient was shown to have a 143% increase of strength during static wrist extension. This result must be interpreted with caution because a double-blind strength-measuring protocol was not used. Furthermore, we have noted that this change slowly decayed over time. The strength of 2 other patients was increased less remarkably (41% and 51%), while the strength of the fourth patient was unchanged.
- Publication
Muscle & Nerve, 1992, Vol 15, Issue 5, p550
- ISSN
0148-639X
- Publication type
Article
- DOI
10.1002/mus.880150504