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- Title
GNOM‐dependent endocytosis maintains polar localisation of the borate exporter BOR1 in Arabidopsis.
- Authors
Yoshinari, Akira; Toda, Yosuke; Takano, Junpei
- Abstract
Background Information: Plants use transporters polarly localised in the plasma membrane for the directional transport of nutrients. The boric acid/borate (B) exporter BOR1 is localised polarly in the inner lateral domain of the plasma membrane in various root cells for efficient translocation of B under B limitation. With a high B supply, BOR1 is ubiquitinated and transported to vacuoles for degradation. The polar localisation and vacuolar targeting of BOR1 are maintained by different endocytosis mechanisms. Results: We demonstrated that one of the most utilised inhibitors in endosomal recycling, brefeldin A (BFA), inhibits the polar localisation of BOR1. BFA inhibits a subset of guanine‐nucleotide exchange factors (ARF‐GEFs), regulators of vesicle formation. Using a transgenic line expressing BFA‐resistant engineered GNOM, we identified GNOM as the key ARF‐GEF in endocytosis and maintenance of the polar localisation of BOR1. Conclusions and Significance: We found that BFA inhibits the polar localisation of BOR1 by inhibiting GNOM activity. Our results suggest that GNOM‐dependent endocytosis contributes to the maintenance of the polar localisation of BOR1 under B limitation. We propose a model of BOR1 transcytosis initiated from GNOM‐dependent endocytosis.
- Subjects
BIOLOGICAL transport; ENDOCYTOSIS; BORATES; EXPORTERS; ARABIDOPSIS; BORIC acid
- Publication
Biology of the Cell (Wiley-Blackwell), 2021, Vol 113, Issue 5, p264
- ISSN
0248-4900
- Publication type
Article
- DOI
10.1111/boc.202000106