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- Title
Resistance training minimizes catabolic effects induced by sleep deprivation in rats.
- Authors
Mônico-Neto, Marcos; Antunes, Hanna Karen Moreira; Lee, Kil Sun; Phillips, Stuart M.; Giampá, Sara Quaglia de Campos; Souza, Helton de Sá; Dáttilo, Murilo; Medeiros, Alessandra; de Moraes, Wilson Max; Tufik, Sergio; de Mello, Marco Túlio
- Abstract
Sleep deprivation (SD) can induce muscle atrophy. We aimed to investigate the changes underpinning SD-induced muscle atrophy and the impact of this condition on rats that were previously submitted to resistance training (RT). Adult male Wistar EPM-1 rats were randomly allocated into 1 of 5 groups: control, sham, SD (for 96 h), RT, and RT+SD. The major outcomes of this study were muscle fiber cross-sectional area (CSA), anabolic and catabolic hormone profiles, and the abundance of select proteins involved in muscle protein synthesis and degradation pathways. SD resulted in muscle atrophy; however, when SD was combined with RT, the reduction in muscle fiber CSA was attenuated. The levels of IGF-1 and testosterone were reduced in SD animals, and the RT+SD group had higher levels of these hormones than the SD group. Corticosterone was increased in the SD group compared with the control group, and this increase was minimized in the RT+SD group. The increases in corticosterone concentrations paralleled changes in the abundance of ubiquitinated proteins and the autophagic proteins LC3 and p62/SQSTM1, suggesting that corticosterone may trigger these changes. SD induced weight loss, but this loss was minimized in the RT+SD group. We conclude that SD induced muscle atrophy, probably because of the increased corticosterone and catabolic signal. High-intensity RT performed before SD was beneficial in containing muscle loss induced by SD. It also minimized the catabolic signal and increased synthetic activity, thereby minimizing the body's weight loss.
- Subjects
ADRENOCORTICAL hormones; ANALYTICAL biochemistry; ANIMAL experimentation; COLLECTION &; preservation of biological specimens; CHEMILUMINESCENCE assay; STATISTICAL correlation; EXERCISE physiology; MUSCLE proteins; MUSCULAR atrophy; PROBABILITY theory; PROTEOLYTIC enzymes; RATS; RESEARCH funding; STATISTICAL sampling; SLEEP deprivation; STATISTICS; T-test (Statistics); WESTERN immunoblotting; DATA analysis; CONTROL groups; REPEATED measures design; DATA analysis software; SKELETAL muscle; DESCRIPTIVE statistics; FLUOROIMMUNOASSAY; RESISTANCE training; ONE-way analysis of variance; PHYSIOLOGY; PREVENTION
- Publication
Applied Physiology, Nutrition & Metabolism, 2015, Vol 40, Issue 11, p1143
- ISSN
1715-5312
- Publication type
Article
- DOI
10.1139/apnm-2015-0061