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- Title
Differential regulation of the release of tumor necrosis factor-α and of eicosanoids by mast cells in rat airways after antigen challenge.
- Authors
Trezena, Aryene Goes; da Silva, Zilma Lucia; Oliveira-Filho, Ricardo Martins; Damazo, Amilcar Sabino; Straus, Anita Hilda; Takahashi, Helio Kiyoshi; Oliani, Sonia Maria; de Lima, Wothan Tavares
- Abstract
B ackground Rat trachea display a differential topographical distribution of connective tissue mast cells (CTMC) and mucosal mast cells (MMC) that may imply regional differences in the release of allergic mediators such as tumor necrosis factor-α (TNF-α) and eicosanoids. Aim To evaluate the role of CTMC and MMC for release of TNF-α and eicosanoids after allergenic challenge in distinct segments of rat trachea. Materials and methods Proximal trachea (PT) and distal trachea (DT) from ovalbumin (OVA)-sensitized rats, treated or not with compound 48/80 (48/80) or dexamethasone, were incubated in culture medium. After OVA challenge, aliquots were collected to study release of TNF-α and eicosanoids. Results Release of TNF-α by PT upon OVA challenge peaked at 90 min and decayed at 6 and 24 h. Release from DT peaked at 30-90 min and decayed 6 and 24 h later. When CTMC were depleted with 48/80, OVA challenge exacerbated the TNF-α release by PT at all time intervals, while DT exacerbated TNF-α levels 6 and 24 h later only. Dexamethasone reduced TNF-α production after 90 min of OVA challenge in PT and at 3 and 6h in DT. OVA challenge increased prostaglandin D 2 in DT and leukotriene B 4 in both segments but did not modify prostaglandin E 2 and leukotriene C 4 release. Conclusion : OVA challenge induces TNF-α release from MMC, which is negatively regulated by CTMC. The profile of TNF-α and eicosanoids depends on the time after OVA challenge and of the tracheal segment considered.
- Subjects
TUMOR necrosis factors; EICOSANOIC acid derivatives; MAST cells
- Publication
Mediators of Inflammation, 2003, Vol 12, Issue 4, p237
- ISSN
0962-9351
- Publication type
Article
- DOI
10.1080/09629350310001599684