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- Title
CD68 Positive Epidermal Dendritic Cells.
- Authors
Petzelbauer, Peter; Födinger, Dagmar; Rappersberger, Klemens; Volc-Platzer, Beatrix; Wolff, Klaus
- Abstract
In a pilot study designed to investigate immunopathologic events in the evolution of cutaneous lesions in pemphigus foliaceus, we found that in this condition the epidermis is replete with CD68+ dendritic cells. The present study was designed to investigate the nature of this novel intraepidermal CD68+cell population. For that purpose lesional skin of five patients with PF and, for comparison, of patients with another acantholytic autoimmune disease, pemphigus vulgaris, were examined using a panel of monoclonal antibodies in a three-step immunoperoxidase technique, in an immunofluorescence double-labeling technique, and by immunoelectron microscopy. We found epidermal CD1a+ Langerhans cells significantly decreased in pemphigus foliaceus compared to pemphigus vulgaris, but pemphigus foliaceus and not pemphigus vulgaris epidermis harbored large amounts of bone marrow - derived (CD45+) cells that expressed CD68, HLA-DR, and β-integrin antigens, the most pronounced expression being observed for CD11c and CD18. These epidermal CD68+ cells were of dendritic shape, were CD1-, and lacked Birbeck granules (BG); however, a small portion of CD68+ cells was also CD1a+ and exhibited BG as revealed by immunoelectron microscopy. These findings demonstrate that in certain conditions, i.e., in pemphigus foliaceus but not in pemphigus vulgaris, there is a shift from CD1a+/CD68- epidermal Langerhans cells towards CD1-/CD68+ dendritic epidermal cells. The detection of a small number of CD1a+/CD68+/BG+ dendritic epidermal cells may identify these cells as a link between the CD1a+/CD68+/BG+ Langerhans cells and the CD1a-/ CD68+/BG- cell population and suggests that these cells represent a transitional form of myelomonocytic cells during their phenotypic and morphologic transformation into resident epidermal Langerhans cells.
- Subjects
IMMUNOPATHOLOGY; PEMPHIGUS; EPIDERMIS; DENDRITIC cells; AUTOIMMUNE diseases; IMMUNOGLOBULINS
- Publication
Journal of Investigative Dermatology, 1993, Vol 101, Issue 3, p256
- ISSN
0022-202X
- Publication type
Report
- DOI
10.1111/1523-1747.ep12365183