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- Title
Mesenchymal Stem Cells Enhance Wound Healing Through Differentiation and Angiogenesis.
- Authors
Yaojiong Wu; Liwen Chen; Scott, Paul G.; Tredget, Edward E.
- Abstract
Although chronic wounds are common, treatment for these disabling conditions remains limited and largely ineffective. In this study, we examined the benefit of bone marrowderived mesenchymal stem cells (BM-MSCs) in wound healing. Using an excisional wound splinting model, we showed that injection around the wound and application to the wound bed of green fluorescence protein (GFP)+ allogeneic BM-MSCs significantly enhanced wound healing in normal and diabetic mice compared with that of allogeneic neonatal dermal fibroblasts or vehicle control medium. Fluorescenceactivated cell sorting analysis of cells derived from the wound for GFP-expressing BM-MSCs indicated engraftments of 27% at 7 days, 7.6% at 14 days, and 2.5% at 28 days of total BM-MSCs administered. BM-MSC-treated wounds exhibited significantly accelerated wound closure, with increased re-epithelialization, cellularity, and angiogenesis. Notably, BM-MSCs, but not CD34+ bone marrow cells in the wound, expressed the keratinocyte-specific protein keratin and formed glandular structures, suggesting a direct contribution of BM-MSCs to cutaneous regeneration. Moreover, BM-MSCconditioned medium promoted endothelial cell tube formation. Real-time polymerase chain reaction and Western blot analysis revealed high levels of vascular endothelial growth factor and angiopoietin-1 in BM-MSCs and significantly greater amounts of the proteins in BM-MSC-treated wounds. Thus, our data suggest that BM-MSCs promote wound healing through differentiation and release of proangiogenic factors.
- Subjects
HEMATOPOIETIC stem cells; BONE marrow; MARMOSA; OSTEOCLASTS; GASTROINTESTINAL stromal tumors; ENDOTHELIAL seeding; GENE expression
- Publication
Stem Cells, 2007, Vol 25, Issue 10, p2648
- ISSN
1066-5099
- Publication type
Article
- DOI
10.1634/stemcells.2007-0226