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- Title
Regulation and Functional Involvement of Distinct Determinants of Leucocyte Function-Associated Antigen 1 (LFA-1) in T-Cell Activation In Vitro.
- Authors
Desroches, C. Vermot; Rigal, D.; Andréoni, C.
- Abstract
The expression of leucocyte function-associated antigen 1 (LFA-1) was studied by immunofluorescence method on human peripheral blood mononuelcar cells (PBMC) stimulated by the phytohaemagglutinin lectin (PHA). Monoclonal antibodies (MoAb) Mas 191c, IOT18 (directed against the β-chain, 95 kDa, CD18) and IOT16, SPVL7, MHM24 (identificating the α-chain, 180 kDa, CD11a) were used, defining the 'CD11a/CD18' antibody panel. By means of cross-linking or competitive experiments, we showed that these antibodies recognized at least four distinct and spatially distant domains on the LFA-1 molecule. Immunofluorescence analysis revealed that the up-regulation of LFA-1 expression was a late event, similar lo the expression kinetics of the HLA DR and CD38 molecules, and followed the appearance of CD25 and CD71 molecules. Moreover, it was established that the LFA-1 upregulation required mRNA and protein synthesis. Functional activity comparison of the different anti LFA-1 MoAb showed that the CD11a MoAb significantly inhibited the proliferation of lymphocytes stimulated by the phytohaemagglutinin to various extents, as the LFA-1α determinant identified. By contrast, the CD18 MoAb did not influence strongly this cell process. We observed only a dim inhibitory effect with the CD18 MoAb recognizing an epitope common or very close to an LFA-1 α determinant. These results suggested that the LFA-1 antigen was important, at a molecular level, in the regulation of T-cell activation.
- Subjects
IMMUNOGLOBULINS; FLUORESCENCE; IMMUNOFLUORESCENCE; MOLECULAR cloning; MONOCLONAL antibodies; MESSENGER RNA
- Publication
Scandinavian Journal of Immunology, 1991, Vol 33, Issue 3, p277
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01773.x