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- Title
In Vitro and in Vivo Stimulation of Murine Lymphocytes by Human Respiratory Syncytial Virus Strains.
- Authors
Alsheikhly, A.-R.; Norrby, E.
- Abstract
Respiratory syncytial virus (RSV) strains of subtype A (A2, WV9894. and WVI2138) and of subtype B (WV1293, WV4843. and WV6873) are mitogenic in vitro for unprimed BALB/c spleen cells. The virus also triggered splenocytes in vitro to secrete immunoglobulins. Plaque-purified and UV-irradiated materials of both RSV subtypes produced comparable levels of DNA synthesis. Infectious materials of both subtypes also induced pronounced responses. Lymphocyte activation with UV-inactivated RSV strain A; was dose-dependent and maximal responses occurred after 4-5 days of incubation. The virus preparations were mitogenic for spleen cells depleted of T lymphocytes by treatment with anti-Thy 1.2 and complement and for lymphocytes of congenitally athymic mice (nu-nu). They were also mitogenic for highly purified T lymphocytes separated by panning of spleen cells on anti-mouse Ig-coated Petri dishes. suggesting that both B and T lymphocytes respond to the mitogenic activity of RSV. Moreover, mice infected intranasally with RSV strain A2 generated local was well as peripheral cellular and humoral responses.
- Subjects
RESPIRATORY syncytial virus; LYMPHOCYTES; IMMUNOGLOBULINS; DNA synthesis; T cells; LABORATORY mice
- Publication
Scandinavian Journal of Immunology, 1987, Vol 26, Issue 2, p161
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1987.tb02248.x