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- Title
Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase.
- Authors
Liu, Yuanzhi; Li, Yanglin; Wang, Mingshu; Cheng, Anchun; Ou, Xumin; Mao, Sai; Sun, Di; Wu, Ying; Yang, Qiao; Jia, Renyong; Tian, Bin; Zhang, Shaqiu; Zhu, Dekang; Chen, Shun; Liu, Mafeng; Zhao, Xinxin; Huang, Juan; Gao, Qun; Yu, Yanling; Zhang, Ling
- Abstract
Background: Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1. Methods: In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cell cycle of duck embryo fibroblasts (DEFs). Subsequently, we analyzed the effects of cell cycle phases on DHAV-1 replication by real-time reverse transcriptase quantitative PCR (real-time RT-qPCR). Results: Flow cytometry data analysis found that DEFs in the S phase increased by 25.85% and 54.21% at 24 h and 48 h after DHAV-1 infection, respectively. The levels of viral RNA detected by real-time RT-qPCR were higher in the DEFs with synchronization in the S phase or G0/G1 phase than in the control group. However, there was no difference in viral copy number between the G2/M phase arrest and control groups. In addition, non-structural protein 3D of DHAV-1 significantly increased cells in the S phase, indicating that 3D protein is one of the reasons for the cell cycle arrest in the S phase. Conclusions: In summary, DHAV-1 infection induces the cell cycle arrest of DEFs in the S phase. Both S phase and G0/G1 phase synchronization facilitate the replication of DHAV-1, and 3D protein is one of the reasons for the S phase arrest.
- Subjects
HEPATITIS A virus; HEPATITIS viruses; VIRAL hepatitis; CELL cycle regulation; DUCK plague; CELL cycle
- Publication
Virology Journal, 2022, Vol 19, Issue 1, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/s12985-022-01839-6