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- Title
Enhanced Assessment of Cross-Reactive Antigenic Determinants within the Spike Protein.
- Authors
Lechuga, Guilherme C.; Temerozo, Jairo R.; Napoleão-Pêgo, Paloma; Carvalho, João P. R. S.; Gomes, Larissa R.; Bou-Habib, Dumith Chequer; Morel, Carlos M.; Provance Jr., David W.; Souza, Thiago M. L.; De-Simone, Salvatore G.
- Abstract
Despite successful vaccination efforts, the emergence of new SARS-CoV-2 variants poses ongoing challenges to control COVID-19. Understanding humoral responses regarding SARS-CoV-2 infections and their impact is crucial for developing future vaccines that are effective worldwide. Here, we identified 41 immunodominant linear B-cell epitopes in its spike glycoprotein with an SPOT synthesis peptide array probed with a pool of serum from hospitalized COVID-19 patients. The bioinformatics showed a restricted set of epitopes unique to SARS-CoV-2 compared to other coronavirus family members. Potential crosstalk was also detected with Dengue virus (DENV), which was confirmed by screening individuals infected with DENV before the COVID-19 pandemic in a commercial ELISA for anti-SARS-CoV-2 antibodies. A high-resolution evaluation of antibody reactivity against peptides representing epitopes in the spike protein identified ten sequences in the NTD, RBD, and S2 domains. Functionally, antibody-dependent enhancement (ADE) in SARS-CoV-2 infections of monocytes was observed in vitro with pre-pandemic Dengue-positive sera. A significant increase in viral load was measured compared to that of the controls, with no detectable neutralization or considerable cell death, suggesting its role in viral entry. Cross-reactivity against peptides from spike proteins was observed for the pre-pandemic sera. This study highlights the importance of identifying specific epitopes generated during the humoral response to a pathogenic infection to understand the potential interplay of previous and future infections on diseases and their impact on vaccinations and immunodiagnostics.
- Subjects
SARS-CoV-2; COVID-19; GLYCOPROTEIN synthesis; EPITOPES; CORONAVIRUSES
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 15, p8180
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25158180