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- Title
FREE RADICAL-SCAVENGING ACTIVITIES OF LOW MOLECULAR WEIGHT CHITIN OLIGOSACCHARIDES LEAD TO ANTIOXIDANT EFFECT IN LIVE CELLS.
- Authors
DAI-NGHIEP NGO; MOON-MOO KIM; ZHONG-JI QIAN; WON-KYO JUNG; SANG-HOON LEE; SE-KWON KIM
- Abstract
Chitin oligosaccharides (NA-COS) with low molecular weight distribution of 229.21–593.12 Da were produced from crab chitin by acid hydrolysis. They showed reducing power and scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) (Sigma Chemical Co., St. Louis, MO), hydroxyl and alkyl radicals. It was observed that the radical-scavenging activity of NA-COS increased in a dose-dependent manner. Their IC50 values for DPPH, hydroxyl and alkyl radicals were 0.8, 1.75 and 1.14 mg/mL, respectively. Furthermore, NA-COS exhibited the inhibitory effect on the oxidative damage of DNA from human lymphoma U937 (American Type Culture Collection, Manassas, VA) and the direct radical-scavenging effect in human fibrosarcoma cells (HT1080) (American Type Culture Collection) in 2′,7′-dichlorofluorescin diacetate (DCFH-DA) assay (Molecular Probes Inc., Eugene, OR). The results suggest that NA-COS can exert antioxidant effect in live cells and have the potential to be applied to food supplements or nutraceuticals. PRACTICAL APPLICATIONS Chitin oligosaccharides (NA-COS) are the hydrolyzed products of chitin (KEUMHO chemical products Co. Ltd., Gyeongbuk, Korea) of which derivatives have shown antioxidant, antimicrobial, anticancer, anti-inflammatory and immunostimulant effects. According to previous studies, NA-COS have beneficial biological activities similar to those of chitin. Furthermore, they are easily soluble in water because of their shorter chain length. Therefore, NA-COS are potentially applicable to improve food quality and human health.
- Subjects
CHITIN; OLIGOSACCHARIDES; MOLECULAR weights; DNA damage; LYMPHOMAS
- Publication
Journal of Food Biochemistry, 2010, Vol 34, p161
- ISSN
0145-8884
- Publication type
Article
- DOI
10.1111/j.1745-4514.2009.00321.x