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- Title
Epstein-Barr virus induces an oxidative stress during the early stages of infection in B lymphocytes, epithelial, and lymphoblastoid cell lines.
- Authors
Saloua Lassoued; Randa Ben Ameur; Wajdi Ayadi; Bochra Gargouri; Riadh Ben Mansour; Hammadi Attia
- Abstract
Abstract The study investigates the direct effect of Epstein-Barr virus infection on the oxidative profile of in vitro cultivated human cells. For this purpose, a panel of human EBV target cells presenting heterogeneity in their cellular and culture types (epithelial cells or lymphocytes; primary culture or continuous cell culture) was selected. These cells are purified human B lymphocytes, DG75, 293, and HepG2 cell lines. The oxidative stress was evaluated during the early stages of infection (2, 12, and 24 h) by measuring malondialdehyde, the end product of the lipid peroxidation, as well as the activities of two antioxidant enzymes: catalase and superoxide dismutase. The obtained results were compared with those of the untreated cells and the K562 cell line which has no interaction with EBV. The incubation of the different target cells with EBV induced an oxidative stress in the purified B lymphocytes, DG75, and 293, but not in HepG2 and K562. This oxidative stress was highlighted by an increase in MDA level (P P
- Subjects
EPSTEIN-Barr virus; B cells; EPITHELIAL cells; LYMPHOBLASTOID cell lines; DISEASES
- Publication
Molecular & Cellular Biochemistry, 2008, Vol 313, Issue 1/2, p179
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-008-9755-z