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- Title
Application of UPLC-QTOF-MS in MS mode for the rapid and precise identification of alkaloids in goldenseal ( Hydrastis canadensis).
- Authors
Le, Phuong; McCooeye, Margaret; Windust, Anthony
- Abstract
Here, we describe a new application of ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MS mode (UPLC-QTOF-MS) for the sensitive, fast, and effective characterization of alkaloids in goldenseal ( Hydrastis canadensis). This approach allowed identification of alkaloids using a cyclic low and high collision energy spectral acquisition mode providing simultaneous accurate precursor and fragment ion mass information. A total of 45 compounds were separated and 40 of them characterized including one new compound and 7 identified for the first time in goldenseal. The spectral data obtained using this method is comparable to those obtained by conventional LC-MS. However, the UPLC-QTOF-MS method offers high chromatographic resolution with structural characterization facilitated by accurate mass measurement in both MS and MS/MS modes in a single analytical run; this makes it suitable for the rapid analysis and screening of alkaloids in plant extracts. [Figure not available: see fulltext.]
- Subjects
LIQUID chromatography; ELECTROSPRAY ionization mass spectrometry; TIME-of-flight mass spectrometry; GOLDENSEAL; CHROMATOGRAPHIC analysis
- Publication
Analytical & Bioanalytical Chemistry, 2014, Vol 406, Issue 6, p1739
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-013-7558-x