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- Title
Introgression of bread wheat chromatin into tall wheatgrass via somatic hybridization.
- Authors
Haifeng Cui; Zhiyong Yu; Jingyao Deng; Xin Gao; Yang Sun; Guangmin Xia
- Abstract
Regenerates were obtained following somatic hybridization between tall wheatgrass ( Agropyron elongatum) and bread wheat ( Triticum aestivum cv. Jinan177) protoplasts. Two lines (CU and XI) were self-fertile in the first (R0) and subsequent (R1 and R2) generations. The phenotype of each R1 population was uniform. All CU progeny were phenotypically similar to the tall wheatgrass parent, while XI progeny had thinner, smoother and softer leaves. Cytological analysis showed that more wheat chromatin was present in the hybrid callus than in the R1 and R2 plants, and that some intercalary translocations of wheat chromosome segments were retained in the R2 generation. AFLP profiling confirmed the presence of wheat DNA in the introgression lines. Analysis of the high molecular weight glutenin subunit content of derived seed identified three novel subunits, not present in either the wheat or the tall wheatgrass parent. Microsatellite-based profiling of the chloroplast genome of the introgression lines suggested that only chloroplast sequences from the tall wheatgrass parent were present. The specifically inherited phenomena and possible application of these hybrids are discussed.
- Subjects
SPECIES hybridization; WHEATGRASS (Wheat); PROTOPLASTS; PHENOTYPES; GENETICS; LEAVES; CHROMATIN; CHROMOSOMES; CALLUS (Botany); DNA
- Publication
Planta: An International Journal of Plant Biology, 2008, Vol 229, Issue 2, p323
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-008-0832-z