We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Increased triacylglycerol production in Rhodococcus opacus by overexpressing transcriptional regulators.
- Authors
Anthony, Winston E.; Geng, Weitao; Diao, Jinjin; Carr, Rhiannon R.; Wang, Bin; Ning, Jie; Moon, Tae Seok; Dantas, Gautam; Zhang, Fuzhong
- Abstract
Lignocellulosic biomass is currently underutilized, but it offers promise as a resource for the generation of commercial end-products, such as biofuels, detergents, and other oleochemicals. Rhodococcus opacus PD630 is an oleaginous, Gram-positive bacterium with an exceptional ability to utilize recalcitrant aromatic lignin breakdown products to produce lipid molecules such as triacylglycerols (TAGs), which are an important biofuel precursor. Lipid carbon storage molecules accumulate only under growth-limiting low nitrogen conditions, representing a significant challenge toward using bacterial biorefineries for fuel precursor production. In this work, we screened overexpression of 27 native transcriptional regulators for their abilities to improve lipid accumulation under nitrogen-rich conditions, resulting in three strains that accumulate increased lipids, unconstrained by nitrogen availability when grown in phenol or glucose. Transcriptomic analyses revealed that the best strain (#13) enhanced FA production via activation of the β-ketoadipate pathway. Gene deletion experiments confirm that lipid accumulation in nitrogen-replete conditions requires reprogramming of phenylalanine metabolism. By generating mutants decoupling carbon storage from low nitrogen environments, we move closer toward optimizing R. opacus for efficient bioproduction on lignocellulosic biomass.
- Subjects
LIGNOCELLULOSE; RHODOCOCCUS; DELETION mutation; OLEOCHEMICALS; GRAM-positive bacteria; WHEAT straw; LIGNINS
- Publication
Biotechnology for Biofuels & Bioproducts, 2024, Vol 17, Issue 1, p1
- ISSN
2731-3654
- Publication type
Article
- DOI
10.1186/s13068-024-02523-3