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- Title
The adipogenesis inhibition of marine microalgae fatty acid extract in hepatocytes.
- Authors
Wang, J. X.; Chu, J. H.; Lin, C. S.; Wu, H. T.
- Abstract
Isochrysis galbana is a species of Haptophyta, which can synthesize unsaturated fatty acids such as EPA and DHA, and rich content in vitamins, polysaccharides, sterols and carotenoids. Therefore, it is one of the valuable sources for human and animal nutrition. Hydrogen peroxide (H2O2) is a reactive oxygen species and the simplest peroxide. Hydrogen peroxide can damage cell DNA and is often used in in vitro studies. In this study, the fatty acid of Isochrysis galbana was extracted and added into H2O2 damage-induced mouse hepatocytes cell line BNL CL.2 in different conditions. To determine the adipogenesis inhibition effect of marine microalgae fatty acid, we analyzed cell activity and antioxidant activity by MTT and DCFDA, and analyzed the expression of proinflammatory cytokines, apoptosis genes, adipogenic transcription genes by RT-PCR. The results showed Isochrysis galbana fatty acid extract can effectively reduce ROS production, protect cells from H2O2 damage. The downward trend of Casp3, and the significantly reduced Fabp4 expression showed Isochrysis galbana fatty acid extract has the effect of restraining apoptosis and lipid accumulation in vitro.
- Subjects
MICROALGAE; ADIPOGENESIS; PRYMNESIOPHYCEAE; UNSATURATED fatty acids; REACTIVE oxygen species
- Publication
Journal of the Chinese Society of Animal Science, 2021, Vol 50, p149
- ISSN
0253-9187
- Publication type
Article