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- Title
Phytochemical Standardization Of Aegle Marmelos Leaves Extract By Using High Performance Thin Layer Chromatography Fingerprinting (Hptlc).
- Authors
Rathod, Sushma; Sihare, Mohini
- Abstract
Objective: Aegle marmelos is well known for its therapeutic properties in the Indian traditional medicinal system. The presence of important bioactive compounds in plants makes them pharmacologically valuable. Therefore, in the present study, the high-performance thin layer chromatography (HPTLC) analysis of hydroalcoholic extract of Aegle marmelos was performed for its phytochemical profiling. Methods: Preliminary phytochemical screening was done. HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, Reprostar 3 and WIN CATS were used. The HPTLC densitometric analysis of the hydroalcoholic extract of Aegle marmelos was carried out using CAMAG HPTLC system, and the results were obtained in the form of chromatograms (scanned at the wavelength of 254 nm and 366 nm) representing several peaks. Results: Preliminary phytochemical screening of the extract showed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, terpenoids, phenolic compounds, proteins, fats and oils. TLC resulted in identification of three spots found in the hydroalcoholic extract. The phytochemical profile of the plant was determined and presented in the tables showing the total number of peaks, peak heights, peak area, percent area, and Rf values. HPTLC finger printing of hydroalcoholic extract of Aegle marmelos under white light reveal presence of five components 0.04, 0.03, 0.29, 0.42, 0.49. Component number 2,3 and 4 at Rf 0.03, 0.29 and 0.42 showed maximum concentration. Conclusion: The study concluded that hydroalcoholic extract of Aegle marmelos contains a rich variety of phytochemicals which might be accountable for its therapeutic value and thus justifies its traditional use in India.
- Publication
Journal of Advanced Zoology, 2024, Vol 45, Issue 2, p801
- ISSN
0253-7214
- Publication type
Article