We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A fluorescence-based method for the detection of adhesive properties of lactic acid bacteria to Caco-2 cells.
- Authors
Bianchi, M. A.; Del Rio, D.; Pellegrini, N.; Sansebastiano, G.; Neviani, E.; Brighenti, F.
- Abstract
m.a. bianchi, d. del rio, n. pellegrini, g. sansebastiano, e. neviani and f. brighenti. 2004. The ability of probiotic micro-organisms to adhere to the intestinal surface is regarded as a substantial advantage in terms of bacteria persistence in the gastrointestinal tract. The aim of the present study was the development of a method based on fluorescent staining of bacteria and subsequent spectrofluorimetric detection to quantify the adhesion of several strains of Lactobacillus and Bifidobacterium to Caco-2 cells. Lactic acid bacteria strains were subjected to fluorescent staining using the viable probe carboxyfluorescein diacetate and subsequently incubated on Caco-2 monolayers. The adhesion of the micro-organisms was determined by spectrofluorimetry following the lysis of the attached bacterial cells and expressed as adhesion percentage. The values obtained for the micro-organisms tested ranged from 4% for Bifidobacterium infantis Bi1 to 10% for a Bifidobacterium mixture containing three different strains. In the present study we successfully applied fluorescent labelling and fluorimetric detection to investigate the adhesive properties of some Lactobacillus and Bifidobacterium strains and a Bifidobacterium mixture to Caco-2 cells. The results proved that fluorescent labelling is suitable for adhesion studies and provides a reliable and safer alternative to radioactive labelling.
- Subjects
BACTERIAL adhesion; CELL adhesion; BIFIDOBACTERIUM; LACTOBACILLUS; LACTIC acid bacteria; LACTOBACILLACEAE; MICROBIOLOGY
- Publication
Letters in Applied Microbiology, 2004, Vol 39, Issue 3, p301
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2004.01589.x