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- Title
Pheophytin a, a low molecular weight compound found in the marine brown alga Sargassum fulvellum, promotes the differentiation of PC12 cells
- Authors
Ina, Atsutoshi; Hayashi, Ken-Ichiro; Nozaki, Hiroshi; Kamei, Yuto
- Abstract
Abstract: We identified and characterized a neurodifferentiation compound from the marine brown alga Sargassum fulvellum collected from the Japanese coastline. Several instrumental analyses revealed the compound to be pheophytin a. Pheophytin a did not itself promote neurite outgrowth of PC12 cells. However, when PC12 cells were treated with a low concentration of pheophytin a (3.9μg/ml) in the presence of a low level of nerve growth factor (10ng/ml), the compound produced neurite outgrowth similar to that produced by a high level of nerve growth factor (50ng/ml). Pheophytin a also enhanced signal transduction in the mitogen-activated protein kinase signaling pathway, which is also induced by nerve growth factor. The effect of pheophytin a on neurite outgrowth of PC12 cells was completely blocked by U0126, a representative mitogen-activated protein kinase kinase inhibitor. These results suggest that pheophytin a enhances the neurodifferentiation of PC12 cells in the presence of a low level of nerve growth factor and that this effect is mediated by activation of a mitogen-activated protein kinase signaling pathway.
- Subjects
SARGASSUM; NERVE growth factor; MITOGEN-activated protein kinases; NERVE tissue proteins
- Publication
International Journal of Developmental Neuroscience, 2007, Vol 25, Issue 1, p63
- ISSN
0736-5748
- Publication type
Article
- DOI
10.1016/j.ijdevneu.2006.09.323