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- Title
Detección de Virus Fitopatógenos en la Región Centro-Norte del Pacífico Mexicano.
- Authors
Antonio Garzón-Tiznado, José; de Jesús Celis-Aramburo, Teresita; Velarde-Félix, Sixto; Ceballos-Ruiz, Julieta; Barbosa-Jasso, Pilar; Reyes-Moreno, Cuauhtémoc; Luis Martinez-Carrillo, José; Sánchez-Peña, Pedro; Hernández-Verdugo, Sergio
- Abstract
The presence of aphid transmitted virus in northwestern Mexico was reported at the end of the 1950's; sixteen years later, the symptoms of leaf rolling and yellowing in tomato plants were described, thereafter, it was determined that geminivirus transmitted by the tobacco whitefly Bemisia tabaci were the causal agents. In order to determine the current status of pathogenic virus in the central and northern parts of the Mexican pacific region 210 plant samples were collected from various crops between 1998 and 2001. Detection of different virus groups was carried out by the ELISA, as well as by PCR, and Southern hybridization in order to confirm the presence of begomovirus. The results indicated that RNA virus such as tobacco etch, and cucumber mosaic virus, represented the highest risk for horticultural crops, along with with begomovirus. It was also confirmed that in 34% of the positive plant samples, virus were present in mixtures, and that tobacco etch-cucumber mosaic virus was the most frequent mixture (45%). Tomato had a 14% of virus mixtures; while cucurbits and chili pepper had a high incidence of virus mixtures with 56 and 49%, respectively. The presence of geminivirus was confirmed by PCR amplification in two tomato samples, and by the Southern hybridization in eight samples of different crops.
- Subjects
MEXICO; SWEETPOTATO whitefly; ENZYME-linked immunosorbent assay; PLANT viruses; POLYMERASE chain reaction; FOOD crops; IMMUNOENZYME technique; RNA viruses; SOUTHERN blot
- Publication
Revista Mexicana de Fitopatología, 2005, Vol 23, Issue 3, p238
- ISSN
0185-3309
- Publication type
Article