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- Title
miR-26a-5p 调控 SLC26A4 挽救听力减退在耳聋中的作用.
- Authors
丛林海; 汤 勇; 殷家志; 邓 苙; 高慧芳; 方红丽; 李 昕
- Abstract
Objective To explore the effect of miR-26a-5p in regulating SLC26A4 to amiliorate hearing loss, and to detect the expression changes of miR-26a-5p and SLC26A4 in deaf mice and hearing loss mice. Methods A mouse hearing loss model and a mouse deafness model were constructed, and the auditory brainstem response test was used to detect the hearing in the right ear of mice to determine whether the models were successfully constructed. qPCR test was used to detect the relative expression of miR-26a-5p and SLC26A4 after hearing loss and deafness in mice were established. The specific site of miR-26a-5p targeting binding to SLC26A4 was predicted at TargetScan website. The interference and overexpression vector of miR-26a-5p and SLC26A4 were injected,, and the expression changes of miR-26a-5p and SLC26A4 were detected. Results After hearing loss and deafness were established, the expression of miR-26a-5p was significantly decreased (P < 0.05), while the expression of SLC26A4 was significantly increased (P < 0.05). After antagomir injection, miR-26a-5p was significantly down-regulated (P = 0.047),while SLC26A4 was significantly up-regulated (P = 0.014);and the hearing threshold of mice decreased significantly (P < 0.05),but the result was just the opposite after injection of miR-26a-5p agomir. Conclusion miR-26a-5p can ameliorate hearing loss through SLC26A4 and thus decelerate the development of deafness.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2023, Vol 44, Issue 6, p14
- ISSN
2095-610X
- Publication type
Article
- DOI
10.12259/j.issn.2095-610X.S20230603