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- Title
Establishment and Application of Sandwich ELISA Method for Detecting Avian Beta-defensin 6.
- Authors
LIANG Rui; LIANG Ying; QI Ke-zong; WANG Ai-ronng; PENG Kai-song
- Abstract
To detect avian beta-defensin 6 (AvBD6) quantitatively, a sandwich ELISA method was established and applied to detect the concentration of AvBD6 in serum. The results showed that the optimal dilution of capture antibody (mouse anti-B cell linear epitopes of AvBD6 polyclonal antibody), detecting antibody (rabbit anti-N-terminal of AvBD6 polyclonal antibody) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG was 2 500, 2 000 and 10 000, respectively. The standard curve regression equation was y =12 024x-2 583, ( R² =0.984 2) in the linear detection range from 20 to 320 ng/mL. The serum AvBD6 concentration of control group was slightly higher than that of ACTH group (P > 0. 05) after 1 day of ACTH (25 IU/kg BW) injection, but the AvBD6 concentration of the control group was slightly lower than that of ACTH group(P>0.05) after 5 d of ACTH injection; and the effect was not significant. The serum AvBD6 concentration of infected group after 24 h of Escherichia coli exposure or 3 h and 24 h of salmonella exposure were significantly higher than that of the control group (P<0.05). Our results indicated that AvBD6 involved in the resistance to system infection of Escherichia coli and salmonella.
- Subjects
ENZYME-linked immunosorbent assay; DEFENSINS; HORSERADISH peroxidase; SALMONELLA; ESCHERICHIA coli
- Publication
Journal of Agricultural Science & Technology (1008-0864), 2014, Vol 16, Issue 4, p176
- ISSN
1008-0864
- Publication type
Article
- DOI
10.13304/j.nykjdb.2014.084