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- Title
Subcellular localization of nuclease in barley aleurone.
- Authors
Holstein, Susanne E. H.; Kobert, Bettina; Hillmer, Stefan; Brown, Peter H.; Ho, T. -H. David; Robinson, David C.
- Abstract
The intracellular localization of an endonuclease (nuclease I) in barley aleurone responding to gibberellic acid was investigated by subcellular fractionation and immunocytochemistry with monoclonal and polyclonal antibodies. Organelle separa- tions were performed with aleurone layers and protoplasts immunefixations were carried out on protoplasts only. Nuclease was detected in fractions from isopycnic sucrose density gradients which were enriched in either endoplasmic reticulum or Golgi apparatus membranes. These two organelles were also labelled by the indirect immunogold method on thin sections. Intensive labelling of protein and developing vacuoles was observed. Therefore, as noted in other plants nuclease in barley is essentially a vacuolar enzyme.
- Subjects
ENDONUCLEASES; GIBBERELLIC acid; NUCLEASES; GOLGI apparatus; IMMUNOGOLD labeling; ENDOPLASMIC reticulum
- Publication
Physiologia Plantarum, 1991, Vol 83, Issue 2, p255
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1991.tb02150.x