We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Noggin upregulates Fos expression by a calcium-mediated pathway in amphibian embryos.
- Authors
Leclerc, Catherine; Duprat, Anne-Marie; Moreau, Marc
- Abstract
In amphibia, noggin, one of the neural inducers expressed in the Spemann organizer, acts by neutralizing the effects of bone morphogenetic protein-4 (BMP-4). It is shown that noggin is able to activate L-type calcium channels. The fos proto-oncogene is known to be induced within minutes by calcium signaling. Here it is reported that in animal cap explants of the amphibian Pleurodeles waltl, noggin can induce upregulation of a FOS-related protein in a calcium-dependent manner. Specific inhibition of the dihydropyridine sensitive L-type calcium channels blocked both calcium influx and the induction of FOS-related protein. When animal cap explants were treated with caffeine in order to release calcium from an internal store or with a specific agonist of the L-type calcium channels, FOS-related protein could be detected in cell nuclei by 5 or 15 min, respectively. Additionally, the calcium calmodulin kinase inhibitor, KN62, could block the upregulation of FOS-related protein induced by agents that increased intracellular calcium ([Ca2+]i). The present results suggest that transcription factors from the FOS family are downstream targets of neural inducer noggin.
- Subjects
FOS oncogenes; CALCIUM channels; AMPHIBIAN eggs
- Publication
Development, Growth & Differentiation, 1999, Vol 41, Issue 2, p227
- ISSN
0012-1592
- Publication type
Article
- DOI
10.1046/j.1440-169x.1999.00421.x