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- Title
Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine.
- Authors
Asam Raza, Muhammad; Kausar, Rukhsana; Ali Rana, Faraz; Danish, Muhammad; Shahwar, Durre; Anwar, Farwa
- Abstract
This study was designed to evaluate the antioxidant potential of Loranthus pulverulentus. Stem bark, leaves, and seeds of Loranthus pulverulentus were extracted in methanol:water (90:10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin-Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ±0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC50 15.9 ± 0.5 µg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09,1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP with R² values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.
- Subjects
ANTIOXIDANTS; NATURAL products; ALTERNATIVE medicine; METHANOL; PLANT extracts
- Publication
Journal of Chemistry, 2013, p1
- ISSN
2090-9063
- Publication type
Article
- DOI
10.1155/2013/250739