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- Title
Evaluation of staining methods for resin embedded cutaneous tissue sections of mycosis fungoides.
- Authors
Chu, A.C.; Smith, N.; MacDonald, D. M.
- Abstract
Biopsies from lesional skin of patients with histologically confirmed mycosis fungoides were embedded in resin and semi-thin (1μm) sections were submitted to a variety of staining procedures, including Toluidine blue, Richardson's, Huber, Giemsa, Paragon and Tribasic stains. The stained sections were examined with the light microscope and the quality of cellular morphological detail afforded by the different stains was assessed. The Toluidine blue, Richardson's and Paragon stains were found to be technically easy to perform and gave the best representation of nuclear and cytopiasmic detail. Of these, the authors favoured Richardson's stain. The technique of processing the tissue, and staining and mounting procedures are detailed.
- Subjects
MYCOSIS fungoides; LYMPHOPROLIFERATIVE disorders; AROMATIC amines; CLINICAL pathology; PATIENTS; DERMATOLOGY
- Publication
British Journal of Dermatology, 1980, Vol 103, Issue 6, p607
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.1980.tb01682.x