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- Title
Mitogen-activated protein kinase/extracellular signal-regulated protein kinase activation of cultured human dental pulp cells by low-power gallium-aluminium-arsenic laser irradiation.
- Authors
Miyata, H.; Genma, T.; Ohshima, M.; Yamaguchi, Y.; Hayashi, M.; Takeichi, O.; Ogiso, B.; Otsuka, K.
- Abstract
Aim,To examine whether low-power laser irradiation (LPLI) promotes cellular proliferation of human dental pulp-derived fibroblast-like cells (dental pulp cells). Methodology,Dental pulp cells were obtained by primary culture of human dental pulp tissues from extracted third molar teeth. The phosphorylation of the mitogen-activated protein kinase (MAPK) family after LPLI of these cells was investigated by Western blotting. By using a specific MAPK/ERK kinase (MEK) inhibitor (PD098059), the specific effect of LPLI on the MAPK pathway was also investigated by Western blotting as described above. The incorporation of [³H]thymidine into the cells after LPLI was determined, and statistical analysis was performed by Wilcoxon signed-ranks test. Results,Extracellular signal-regulated protein kinase (ERK) 1/2 was phosphorylated between 5 and 30 min after LPLI. Moreover, PD098059 inhibited LPLI-mediated ERK1/2 activation. LPLI did not affect p38 MAPK or c-Jun N-terminal kinase (JNK) phosphorylation. But LPLI did not stimulate [³H]thymidine incorporation into these cells. Conclusions,These results indicated that LPLI activated MAPK/ERK, a signal for proliferation, differentiation and survival, but did not activate the stress signals p38 MAPK and JNK in human dental pulp cells.
- Subjects
MITOGEN-activated protein kinases; DENTAL pulp; PYRIMIDINE nucleotides; CELL proliferation; PHOSPHORYLATION; THYMIDINE
- Publication
International Endodontic Journal, 2006, Vol 39, Issue 3, p238
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/j.1365-2591.2006.01080.x