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- Title
Heat shock proteins in Sorghum bicolor and Pennisetum americanum. II. Stored RNA in sorghum seed and its relationship to heat shock protein synthesis during germination.
- Authors
Howarth, C.
- Abstract
During the germination of sorghum, heat shock proteins (HSPs) are synthesized iii response to high-temperature stress at the onset of imbibition. HSP72 and HSP83 are apparently synthesized without a heat shock induction in the first 2 h of imbibition. The presence of RNA encoding HSPs in the quiescent embryo, and in the 2 h imbibed embryo, was confirmed by <em>in vitro</em> translation of RNA isolated from dry mature sorghum seeds. By 24 h of imbibition at 35°C, no translatable RNA was evident for these HSPs unless a heat shock treatment was also given. Treatment of imbibing embryos with cordycepin inhibited RNA synthesis. Protein synthesis was not affected so greatly and HSP synthesis was still inducible although at reduced levels. The involvement of RNA stored in the seed and of <em>de novo</em> transcription in protein synthesis during imbibition is discussed.
- Subjects
GERMINATION; PROTEIN synthesis; SORGHUM; HEAT shock proteins; RNA; PEARL millet
- Publication
Plant, Cell & Environment, 1990, Vol 13, Issue 1, p57
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1111/j.1365-3040.1990.tb01299.x