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- Title
Seed transmission of Melon necrotic spot virus and efficacy of seed-disinfection treatments.
- Authors
Herrera-Vásquez, J. A.; Córdoba-Sellés, M. C.; Cebrián, M. C.; Alfaro-Fernández, A.; Jordá, C.
- Abstract
Rates of seed transmission of Melon necrotic spot virus (MNSV) were estimated in seedlings grown from commercial melon ( Cucumis melo) cv. Galia F1 seeds. Seedlings at the cotyledon stage and adult plants were assayed for MNSV by DAS-ELISA and RT-PCR. None of the seedling groups tested positive for MNSV by ELISA. The proportion of seedlings infected with MNSV was at least 7 and 8% in seed lots 05 and 06, respectively, as estimated from RT-PCR analysis of grouped seedlings. Fourteen and eight grouped samples (10 seedlings per group), of a total of 200 and 100 seedlings, respectively, grown from infected seeds were MNSV-positive in seed lots 05 and 06, respectively, corresponding to seed-to-seedling transmission rates of 11·3 and 14·8%, respectively. Several seed-disinfection treatments were evaluated for their ability to prevent seed transmission of MNSV. The results suggest that a treatment of 144 h at 70°C can be used to eradicate MNSV in melon seeds without hindering germination.
- Subjects
MUSKMELON; SEED pathology; DISINFECTION &; disinfectants; GERMINATION; CARMOVIRUSES; SEEDLINGS; MICROBIOLOGICAL assay
- Publication
Plant Pathology, 2009, Vol 58, Issue 3, p436
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.2008.01985.x