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- Title
Mouse spontaneous mammary adenocarcinoma as a suitable model of breast cancer in Iran.
- Authors
Alizadeh, Ali Mohammad; Azizian, Saleh; Fayazi, Hamid Reza; Mousavi, Maryam-Sadat; Nabavi, Seyed Ali
- Abstract
Background: Breast cancer is a complicated disease and has various clinical manifestations and prognosis. The aim of this study is to make a breast cancer model suitable for basic, preclinical and clinical studies. Methods: Five virgin Bulb/c mice aged 6 weeks and spontaneous Murine Mammary Adenocarcinoma Cells (MMAC) were used. After anesthesia induction, 1-3 million of MMACs were subcutaneously injected into the mice's right lank. Four weeks after the tumor induction, tumor size in both length and width were weekly measured using caliper for 4 weeks. Histopathology studies were done 2 months later by Scraff-Boom-Richard- son grading. Results: Visible tumors were appeared after 4 weeks. Take-rate of all cells was nearly 100%. Tumor sizes reached 0.6±0.1 mm3, 1.1±0.3, 1.68±0.5 and 3±0.7 cm3 with at ifth, sixth, seventh and eighth weeks respectively. The histopathological studies using H & E method showed invasive ductal carcinoma, grade II/III. Conclusion: Elucidation of the molecular mechanisms of breast cancer progression and metastasis has extremely gained from mouse models in which some stages of tumor progression are recapitulated.
- Subjects
BREAST cancer research; BREAST cancer prognosis; CANCER cells; ADENOCARCINOMA; CANCER invasiveness; METASTASIS
- Publication
Basic & Clinical Cancer Research, 2011, Vol 3, Issue 3/4, p30
- ISSN
2228-6527
- Publication type
Article