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- Title
EFECTO DE LA LECHE SOBRE LA CAPACIDAD DE FORMACIÓN DE BIOPELÍCULAS DE Staphylococcus spp. EN ACERO INOXIDABLE.
- Authors
CIRONE SILVA, Nathália Cristina; CRUZADO BRAVO, Melina Luz Mary; RODRIGUEZ, Marjory Xavier; VÁZQUEZ-SÁNCHEZ, Daniel; CONTRERAS CASTILLO, Carmen Josefina
- Abstract
Background: Staphylococcus spp. are able to adhere and form biofilms on several food-contact surfaces. In the dairy industry, milk can be a contamination vector and a nutrient source relevant to the development of Staphylococcus spp. biofilms. Objectives: The present study aimed to evaluate the effect of UHT milk on biofilm-forming ability of Staphylococcus spp. on stainless steel surfaces. Methods: Two S. aureus strains isolated from raw milk (Sta1) and fresh cheese (Sta2) respectively, as well as S. epidermidis ATCC 12228 and S. aureus ATCC 12600 were tested. One sterile stainless steel coupon of 2.7 cm2 was placed into each well of a sterile 24-well flat-bottom microplate. An aliquot of 1 mL of UHT milk or BHI contaminated with 107 CFU·mL-1 was added into each well with a coupon. Microplates were incubated for 12 and 24 h at 25°C. Afterwards, three coupons of the same treatment were immersed in 4 mL of peptone and ultrasonicated for 3 min at 40 kHz to resuspend the adhered cells. Ten-fold serial dilutions of resuspended cells were made in peptone water and aliquots of 0.1 mL of appropriate dilutions were spread on Baird Parker plates. Plates were incubated at 37°C for 48 h and the number of CFU·cm2 calculated. Three independent replicates were done for each strain, culture media and time. Results: No significant differences were found between the growth in milk and BHI. Thus, the biofilm-forming ability of Staphylococcus spp. was similar in both culture media. A fast biofilm formation on stainless steel was observed in all strains contaminating milk, because no significant differences were found between biofilms formed for 12 and 24 h. Strains isolated from dairy products showed a significantly (P<0,05) higher biofilm-forming ability than the reference strains, reaching 8.8 and 8.6 log CFU·cm-2. Conclusions: Milk generates an adequate conditions to the development of biofilms on stainless steel by bacterial pathogens such as Staphylococcus spp. Therefore, an optimization of sanitizing procedures in dairy-processing plants is recommended to avoid the presence and accumulation of milk wastes during extended periods.
- Publication
Vitae (01214004), 2016, Vol 23, pS73
- ISSN
0121-4004
- Publication type
Article