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- Title
Highly sensitive simultaneous quantification of buprenorphine and norbuprenorphine in human plasma by magnetic solid-phase extraction based on P pPDA/FeO nanocomposite and high-performance liquid chromatography.
- Authors
Gomar, Fatemeh; Afkhami, Abbas; Madrakian, Tayyebeh
- Abstract
A rapid and sensitive method based on magnetic solid-phase extraction coupled to high-performance liquid chromatography with ultraviolet detection was developed for the simultaneous determination of buprenorphine (BPN) and its major metabolite, norbuprenorphine (N-BPN), in human plasma samples. Poly (para-phenylenediamine)-modified FeO nanoparticles (P pPDA/FeO) were synthesized and used as a magnetic adsorbent for the extraction and preconcentration of BPN and N-BPN in biological samples. The synthesized nanocomposites were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy and vibrating sample magnetometery. An isocratic separation was achieved on a Nova-Pak C18 reversed-phase column using a mobile phase consisting phosphate buffer (pH 3.4) and acetonitrile (50:50, v/v) at a flow rate of 1.0 mL min. The detection was conducted at 280 nm. Under the optimum conditions, the calibration curves for BPN and N-BPN were linear in the ranges 3.0-150.0 and 1.0-120.0 ng mL, respectively. The sensitivity was also high with limit of detection of 0.8 and 0.3 ng mL for BPN and N-BPN in plasma, respectively. The method was successfully applied to the extraction and determination of BPN and N-BPN in human plasma samples with an average recovery of 98.10 and 96.41%, respectively.
- Subjects
BUPRENORPHINE; BLOOD plasma; MAGNETIC fields; SOLID phase extraction; IRON oxides; NANOCOMPOSITE materials; HIGH performance liquid chromatography
- Publication
Journal of the Iranian Chemical Society, 2018, Vol 15, Issue 3, p575
- ISSN
1735-207X
- Publication type
Article
- DOI
10.1007/s13738-017-1257-z