We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Production of very-high-amylose potato starch by inhibition of SBE A and B.
- Authors
Schwall, Gerhard P.; Safford, Richard; Westcott, Roger J.; Jeffcoat, Roger; Tayal, Akash; Shi, Yong-Cheng; Gidley, Michael J.; Jobling, Stephen A.
- Abstract
High-amylose starch is in great demand by the starch industry for its unique functional properties. However, very few high-amylose crop varieties are commercially available. In this paper we describe the generation of very-high-amylose potato starch by genetic modification. We achieved this by simultaneously inhibiting two isoforms of starch branching enzyme to below 1% of the wild-type activities. Starch granule morphology and composition were noticeably altered. Normal, high-molecular-weight amy-lopectin was absent, whereas the amylose content was increased to levels comparable to the highest commercially available maize starches. In addition, the phosphorus content of the starch was increased more than fivefold. This unique starch, with its high amylose, low amylopectin, and high phosphorus levels, offers novel properties for food and industrial applications.
- Subjects
BIOTECHNOLOGY; STARCH; PULLULANASE; BRANCHING (Botany)
- Publication
Nature Biotechnology, 2000, Vol 18, Issue 5, p551
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/75427