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- Title
QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides.
- Authors
Galka, Pierre; Jamez, Elisabeth; Joachim, Gilles; Soumillion, Patrice
- Abstract
Incorporation of synthetic degenerate oligonucleotides into plasmids for building highly diverse genetic libraries requires efficient and quantitative DNA manipulation. We present a fast and seamless method for generating libraries of PCR-synthesized plasmids designed with a degenerate sequence and short overlapping ends. Our method called QuickLib should find many applications in synthetic biology; as an example, we easily prepared genetic libraries of Escherichia coli expressing billions of different backbone cyclic peptides.
- Subjects
OLIGONUCLEOTIDES; PLASMID genetics; CLONING; ESCHERICHIA coli DNA; ESCHERICHIA coli; BACTERIAL genetics; ESCHERICHIA coli transmission
- Publication
PLoS ONE, 2017, Vol 12, Issue 4, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0175146