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- Title
Molecular Detection of Hepatitis E Virus in Layer Chickens: A Possible Public Health Risk in Pakistan.
- Authors
Iqbal, Tahir; Rashid, Umer; Shahzad, Naveed; Afroz, Amber; Malik, Muhammad Faheem; Idrees, Muhammad
- Abstract
Avian Hepatitis E Virus (aHEV) is a single-stranded positive-sense RNA virus causing hepatitissplenomegaly syndrome (HSS) in chickens. To the best of our knowledge the circulation of aHEV in chicken has not been studied in Pakistan so far. Therefore, in the present study, we aimed to isolate and identify aHEV from layer chickens of Pakistan. The bile fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki region of Punjab province, Pakistan, during July to August 2016. The RT-PCR showed the amplifications of selected regions of helicase (186 bps, 2769- 2954 nt) and capsid (280 bps, 5461-5741 nt) domain in the bile fluids of two birds. The histological data demonstrated pathological changes in liver and spleen tissues of layer chickens positive for viral RNA. The extensive phylogenetic analysis on the basis of partial helicase domain (ORF1) and capsid protein (ORF2) revealed clustering of Pakistani aHEV (Pak aHEV) strains with members of Orthohepevirus B species. However, Pak aHEV strains were highly divergent from other known members within Orthohepevirus B suggesting as novel aHEV strains circulating in the population of layer chickens in the country. Detection of HEV in layer chickens may pose public health risk in context of zoonosis and food borne transmission if aHEV emerges as zoonotic HEV.
- Subjects
PAKISTAN; PUNJAB; HEPATITIS E virus; CHICKENS; PUBLIC health; LIVER; AVIAN anatomy
- Publication
Pakistan Journal of Zoology, 2019, Vol 51, Issue 6, p2329
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2019.51.6.2329.2335