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- Title
In vitro Antiviral Activity of Nigella sativa against Peste des Petits Ruminants (PPR) Virus.
- Authors
Aqil, Kiran; Khan, Muti-ur-Rehman; Aslam, Asim; Javeed, Aqeel; Qayyum, Rizwan; Yousaf, Farooq; Yasmeen, Farkhanda; Sohail, Muhammad Luqman; Umar, Sajid
- Abstract
Peste des petits ruminants (PPR) is an important viral disease of sheep, goat and other small ruminants, imparting substantial economic losses. In current study, antiviral activity of six dilutions of Nigella sativa (N. sativa) alcoholic extracts against the PPRV was investigated in vitro. Vero cell lines infected with PPRV were treated with six dilutions alcoholic extracts of N. sativa (200, 100, 50, 25, 12.5 and 6.25 μg/mL). The antiviral effects were judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric (MTT) assay. Plaque reduction assay was used to test the mode of action. Out of six dilutions, three dilutions (50, 25, 12.5 μg/ml) showed significant antiviral activity and cell survival was more than 50%. Two dilutions of N. sativa (200 μg/ml and 100 μg/ml) extracts showed cytotoxicity to Vero cell lines. In the plaque reduction assay, alcoholic extracts of N. sativa (50 μg/ml) significantly reduced the plaques count as compared with the control negative (P<0.05) in all the tested modes of action. The current experiment revealed that the alcoholic extracts of N. sativa (50 μg/ml) could increase the viability of infected cells, and curtail the cytopathic effects of PPRV. N. sativa seed extracts should be explored in field conditions for the control of PPR.
- Subjects
PESTE des petits ruminants; BLACK cumin; ANTIVIRAL agents; CELL-mediated cytotoxicity; IN vitro studies
- Publication
Pakistan Journal of Zoology, 2018, Vol 50, Issue 6, p2223
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2018.50.6.2223.2228