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- Title
Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats.
- Authors
Rycerz, Karol; Krawczyk, Aleksandra; Jaworska-Adamu, Jadwiga; Krawczyk-Marc, Izabela
- Abstract
Introduction. Calretinin (CR) is a protein, which is present in GABAergic neurons and belongs to the calciumbinding proteins family. It may reduce the excitotoxicity phenomenon through its Ca2+ buffering properties. This phenomenon is due to the increase of calcium ions levels caused by the excess of glutamate -- the main excitatory neurotransmitter. The aim of the study was to investigate alterations of calretinin-immunoreactivity in neurons of hippocampal CA1 region and dentate gyrus with hilus in 10 day-old rats treated with monosodium glutamate (MSG). Material and methods. Ten 7 day-old Wistar rats were used. The MSG-group consisted of 5 MSG-treated rats at a dose of 4 g/kg b.w. for 3 consecutive days and the second group consisted of 5 control animals. After euthanasia the brains containing hippocampus were dissected and embedded in paraffin blocks. The immunohistochemical peroxidase-antiperoxydase reaction was performed on tissue sections. The morphometric analyses of CR-immunopositive neurons: density, percentage ratio to the density of all cells and an assessment of digital immunostaining intensity were performed. Results. The distribution of the CR-immunoreactive neurons in the hippocampus was irregular. In the MSGgroup there were single cells, which were more intensely stained than in control animals. Some of cells contained processes of different length. The density of CR-immunopositive cells and their percentage ratio to the density of all cells did not change significantly after MSG treatment. However, there was a statistically significant increase in the staining intensity of CR-immunopositive cells. Conclusions. The obtained results indicate that CR-positive cells in P7-P10 rats are only slightly affected by MSG in CA1 region and dentate gyrus with hilus of the hippocampus. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 4, 281-288)
- Subjects
IMMUNOHISTOCHEMISTRY; ANALYSIS of variance; ANIMAL experimentation; CALCIUM-binding proteins; HIPPOCAMPUS (Brain); MONOSODIUM glutamate; NEURONS; RATS; STATISTICS; DATA analysis; DATA analysis software; DESCRIPTIVE statistics
- Publication
Folia Histochemica et Cytobiologica, 2014, Vol 52, Issue 4, p281
- ISSN
0239-8508
- Publication type
Article
- DOI
10.5603/FHC.a2014.0030