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- Title
Comparative study of visible light polymerized gelatin hydrogels for 3D culture of hepatic progenitor cells.
- Authors
Greene, Tanja; Lin, Tsai‐Yu; Andrisani, Ourania M.; Lin, Chien‐Chi
- Abstract
ABSTRACT Photopolymerization techniques have been widely used to create hydrogels for biomedical applications. Visible light-based photopolymerizations are commonly initiated by type II (i.e., noncleavage-type) photoinitiator in conjunction with a coinitiator. On the other hand, type I photoinitiators (i.e., cleavage type) are rarely compatible with visible light-based initiation due to their limited molar absorbability in the visible light wavelengths. Here, we report visible light initiated orthogonal photoclick crosslinking to fabricate gelatin-norbornene and poly(ethylene glycol)-tetra-thiol hydrogels using either cleavage-type (i.e., lithium acylphosphinate, LAP) or noncleavage-type photoinitiator (i.e., eosin-Y, EY) without the use of a coinitiator. Regardless of the initiator type, the step-growth gelatin-PEG hybrid hydrogels crosslinked and degraded similarly. While both systems exhibited similar cytocompatibility for hepatic progenitor HepaRG cells, gelation initiated by noncleavage-type initiator EY afforded slightly higher degree of hepatic gene expression. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44585.
- Subjects
PHOTOPOLYMERIZATION kinetics; ELECTROMAGNETIC waves; ELECTROMAGNETIC wave propagation; HYDROGELS; EXPANSION microscopy
- Publication
Journal of Applied Polymer Science, 2017, Vol 134, Issue 11, pn/a
- ISSN
0021-8995
- Publication type
Article
- DOI
10.1002/app.44585