We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Fluorescence resonance energy transfer detection methods: Sensitized emission and acceptor bleaching.
- Authors
JIE QIAN; BINGBO YAO; CHUANYUE WU
- Abstract
The present study compared the advantages and disadvantages of fluorescence resonance energy transfer (FRET) determination technologies, namely, sensitized emission (SE) and acceptor bleaching (AB), in order to analyze the applicability of SE and AB for studies investigating particularly interesting new cysteine histidine-rich protein 1 (PINCH1)/integrin-linked kinase (ILK) interaction. HeLa cells were transfected with cyan fluorescent protein (CFP)-PINCH1 and yellow fluorescent protein (YFP)-ILK to establish a PINCH1/ILK interaction examination model. PINCH1/ILK interactions in different parts of the cells were also examined by SE and AB. The FRET determination technologies SE and AB were able to examine PINCH1/ILK interaction. SE was more sensitive for FRET determination and thus had greater reliability. Therefore, SE is highly commended for membrane protein-protein interaction studies.
- Subjects
FLUORESCENCE resonance energy transfer; SENSITIZED fluorescence; PROTEIN research; INTEGRIN-linked kinase; INTERMOLECULAR interactions; HELA cells
- Publication
Experimental & Therapeutic Medicine, 2014, Vol 8, Issue 5, p1375
- ISSN
1792-0981
- Publication type
Article
- DOI
10.3892/etm.2014.1928