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- Title
Nature of the genome of the saprophytic spirochete Spirochaeta aurantia and its ribosomal RNA operons.
- Authors
McLaughlin, Richard; Secko, David M.; Paul, Catherine J.; Kropinski, Andrew M.
- Abstract
Using restriction endonucleases DraI, AseI, and I-CeuI in conjunction with pulsed-field gel electrophoresis, we have shown that Spirochaeta aurantia M1 possesses a circular 3.98-Mb genome. This is the second largest spirochete chromosome yet analyzed. The observation that the latter enzyme cuts in 3 places suggests the presence of 3 copies of the large subunit (23S) rRNA gene (rrl), which was confirmed by Southern hybridizations. The complete sequence of 2 of the ribosomal RNA operons was determined, revealing that their structure resembled that of the typical member of the bacterial superkingdom: rrs (16S; 1561 bp), tRNA, rrl (23S; 2972 bp), and rrf (5S; 110 bp). The S. aurantia rrs–rrl intergenic regions, as with Treponema denticola, contain genes specifying a 73-nt tRNAAla (anticodon TGC) and a 77-nt tRNAIle (anticodon GAT).
- Subjects
SPIROCHAETA; SPIROCHETES; BACTERIA; GENETIC regulation; TRANSFER RNA; GENETICS; ENZYMES; PULSED-field gel electrophoresis; PHASE partition
- Publication
Canadian Journal of Microbiology, 2004, Vol 50, Issue 11, p967
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W04-086