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- Title
Three unlinked gene clusters are involved in clavam metabolite biosynthesis in Streptomyces clavuligerus.
- Authors
Tahian, Kapil; Hyeon Ung Park; Jensen, Susan E.
- Abstract
In Streptomyces clavutigerus. three groups of genes are known to be involved in the biosynthesis of the clavam metabolites. Since antibiotic biosynthetic genes are invariably clustered on the chromosome in prokaryotes, chromosome walking was undertaken in an attempt to show that the three groups of clavam genes would resolve into a single super-cluster when analyzed at larger scale. However, no evidence of linkage between the three groups was obtained. Furthermore, Southern analysis of macro-restriction fragments of genomie DNA separated by pulsed-field gel electrophoresis also indicated that the three groups of genes are not linked. Despite the structural and biosynthetic relatedness of the clavam metabolites, our results suggest that the genes involved in their production lie in three unlinked gene clusters. We believe that this represents the first instance in bacteria of genes involved in the biosynthesis of a single family of antibiotics sharing a common biosynthetic pathway and yet residing in three separate locations on the chromosome.
- Subjects
STREPTOMYCES; GENES; BIOSYNTHESIS; DNA; METABOLITES; PROKARYOTES; PULSED-field gel electrophoresis
- Publication
Canadian Journal of Microbiology, 2004, Vol 50, Issue 10, p803
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W04-070