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- Title
Screening of Ginkgolides-producing Endophytic Fungi and Optimal Study on Culture Condition.
- Authors
Yan Zhu-yun; Luo Jing; Guo Xiao-heng; Zeng Qing-qiu
- Abstract
Strain was screened by analyse of TLC and HPLC-UV in the fermentation liquid of endophytic fungi; the optimum culture medium was chosen by the determination of growth, spores-producing and yield of ginkgolids on different culture medium, and using HPLC-ELSD determined yield of ginkgolids in the fermentation liquid in different time segment. A high yield strain, Aspergillus fumigatus var.fumigatus FG 052, was screened. The results of experimenting on culture conditions of FG 052 strain showed that PDA is the optimum heredity culture medium and Czapek is the optimum ferment culture medium, the maximum amount of mycelia appeared at fermenting for 168 h, the maximum ginkgolides productivity appeared at 144 h and yield reached 0.13 mg/mL, pH is 4.86 here. The strain screened by this study had good stability, the culture medium chosen was low-cost and definite ratio of carbon and nitrogen sources with high yield of ginkgolides which can be chosen culture medium for ginkgolides in large scale.
- Subjects
ENDOPHYTIC fungi; LACTONES; CULTURES (Biology); CARBON; NITROGEN
- Publication
Natural Product Research & Development, 2007, Vol 19, Issue 4, p554
- ISSN
1001-6880
- Publication type
Article