We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Calcium Metabolism Profile in Rat Inner Ear Indicated by MRI After Tympanic Medial Wall Administration of Manganese Chloride.
- Authors
Zou, Jing; Pyykkö, Ilmari
- Abstract
Objectives: To evaluate the efficacy of the novel method for the targeted delivery of Mn++ to the inner ear and monitor calcium metabolism activity in the inner ear. Materials and Methods: Dynamic signal changes of Mn++ in the rat inner ear were followed using T1-weighted magnetic resonance imaging (MRI) after administration of 2.5 µl MnCl2 (500 mM) to the medial wall of the middle ear cavity. Results: Mn++ passed through both the oval and round windows and distributed in the perilymphatic compartments, where it formed bright sharp lines along the fluid-cellular borders 12 minutes post administration and entered the endolymph sufficiently after 45 minutes. After 6 hours, the distribution of Mn++ shifted from a fluid-dominant pattern to a cell-dominant pattern. Mn++ concentrated in the area of the basilar membrane, periphery process, and soma of the spiral ganglion on day 2; became more distinguishable on day 4; declined on day 8; and remained detectable for 16 days post administration. Conclusions: The novel targeted delivery method efficiently introduced Mn++ into the inner ear. The dynamic distribution pattern of Mn++ in the inner ear shown by MRI indicates that this method can be used to monitor calcium metabolism activity in the inner ear.
- Subjects
CALCIUM metabolism; MIDDLE ear anatomy; INNER ear; ANIMAL experimentation; IONS; MAGNETIC resonance imaging; MANGANESE; RATS; RESEARCH funding; T-test (Statistics); ANATOMY
- Publication
Annals of Otology, Rhinology & Laryngology, 2016, Vol 125, Issue 1, p53
- ISSN
0003-4894
- Publication type
Article
- DOI
10.1177/0003489415597916