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- Title
Introgression of broad-spectrum blast resistance gene(s) into cultivated rice (Oryza sativa ssp indica) from wild rice O. rufipogon.
- Authors
Ram, T.; Majumder, N. D.; Mishra, B.; Ansari, M. M.; Padmavathi, G.
- Abstract
Rice blast disease caused by Magnaporthe grisea (Hebert) Barr, is one of the major diseases of the crop. Host specificity as well as genetic instability of rice blast fungus is the major cause for the breakdown of resistance in many varieties over a period of time. Here we report the introgression of broad-spectrum blast resistance gene(s) from Oryza rufipogon into a cultivated rice variety. An accession (Coll-4) of O. rufipogon, highly resistant to blast, was crossed with a susceptible line B 32-Sel-4 and the F1 was top-crossed with another susceptible line B 29-6. The F2 population of the three-way cross was screened for blast and salinity tolerance. Twenty selected plants, each with blast resistance and salinity tolerance, were intermated. The process of intermating was repeated in the F2 progenies derived from the first intermated populations. After two cycles of intermating, pedigree selections in the resulting segregating generations were followed under saline conditions. The 42 introgression lines (F5 generation) were screened for blast reaction under artificial inoculation. Eight lines were observed to be immune, 20 resistant and six lines moderately resistant to an isolate of blast isolated in Andamans. The remaining eight lines, the two indica rice parents and check CO39 were susceptible. Two promising lines with higher yield potential were evaluated in multilocation trials (AICRIP) during 1999, 2000 and 2001 at 17 hotspot locations for blast infection. The culture B 90-15 (IET 15420) showed resistance reaction against 14 isolates and moderate resistance against another two isolates of blast indicating the introgression of a broad spectrum of resistance to blast from O. rufipogon.
- Subjects
RICE blast disease; PYRICULARIA grisea; DISEASE resistance of plants; PLANT immunology; PLANT breeding research; SALINITY
- Publication
Current Science (00113891), 2007, Vol 92, Issue 2, p225
- ISSN
0011-3891
- Publication type
Article